Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton
Identifieur interne : 001790 ( Main/Exploration ); précédent : 001789; suivant : 001791Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton
Auteurs : YU ZHONG [Japon] ; Yoshiyuki Yoshinaka [Japon] ; Tadahiro Takeda [Japon] ; Noriko Shimizu [Japon] ; Sayaka Yoshizaki [Japon] ; Yoshio Inagaki [Japon] ; Shinobu Matsuda [Japon] ; Gisho Honda [Japon] ; Nobutaka Fujii [Japon] ; Naoki Yamamoto [Japon]Source :
- Antiviral research [ 0166-3542 ] ; 2005.
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Abstract
A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (Ills) infection in MT-4 cells with EC50 values of 0.5 μg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC50 > 1000 μg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC50 of 11.56 μg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 μg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 °C in 1N HCl or IN NaOH, and after treatment with 100mM NaIO4. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton</title><author><name sortKey="Yu Zhong" sort="Yu Zhong" uniqKey="Yu Zhong" last="Yu Zhong">YU ZHONG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Human Gene Sciences Center, Tokyo Medical and Dental University</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>2 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Kyoritsu University of Pharmacy</s1><s2>Minato-ku, 105-8512, Tokyo</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Kyoritsu University of Pharmacy</s1><s2>Minato-ku, 105-8512, Tokyo</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>National Institute of Infectious Diseases</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>5 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Institute of Hemorheological Function of Food Co. 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Ltd</wicri:noRegion></affiliation></author><author><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Graduate School of Pharmaceutical Sciences, Kyoto University</s1><s2>Sakyo-ku, Kyoto 606-8501</s2><s3>JPN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Graduate School of Pharmaceutical Sciences, Kyoto University</s1><s2>Sakyo-ku, Kyoto 606-8501</s2><s3>JPN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>National Institute of Infectious Diseases</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>5 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">05-0266877</idno><date when="2005">2005</date><idno type="stanalyst">PASCAL 05-0266877 INIST</idno><idno type="RBID">Pascal:05-0266877</idno><idno type="wicri:Area/PascalFrancis/Corpus">000047</idno><idno type="wicri:Area/PascalFrancis/Curation">000012</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000049</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000049</idno><idno type="wicri:doubleKey">0166-3542:2005:Yu Zhong:highly:potent:anti</idno><idno type="wicri:Area/Main/Merge">001801</idno><idno type="wicri:Area/Main/Curation">001790</idno><idno type="wicri:Area/Main/Exploration">001790</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Highly potent anti-HIV-1 activity isolated from fermented Polygonum tinctorium Aiton</title><author><name sortKey="Yu Zhong" sort="Yu Zhong" uniqKey="Yu Zhong" last="Yu Zhong">YU ZHONG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Human Gene Sciences Center, Tokyo Medical and Dental University</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>2 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Kyoritsu University of Pharmacy</s1><s2>Minato-ku, 105-8512, Tokyo</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Kyoritsu University of Pharmacy</s1><s2>Minato-ku, 105-8512, Tokyo</s2><s3>JPN</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>National Institute of Infectious Diseases</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>5 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Institute of Hemorheological Function of Food Co. Ltd</s1><s2>Hyogo</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Institute of Hemorheological Function of Food Co. Ltd</wicri:noRegion></affiliation></author><author><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Graduate School of Pharmaceutical Sciences, Kyoto University</s1><s2>Sakyo-ku, Kyoto 606-8501</s2><s3>JPN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name><affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Graduate School of Pharmaceutical Sciences, Kyoto University</s1><s2>Sakyo-ku, Kyoto 606-8501</s2><s3>JPN</s3><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName><orgName type="university">Université de Kyoto</orgName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Molecular Virology, Bio-Response, Graduate School, Tokyo Medical and Dental University, 1-5-45, Yushima</s1><s2>Bunkyo-ku, Tokyo 113-8519</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>National Institute of Infectious Diseases</s1><s2>Shinjuku-ku, Tokyo 162-8640</s2><s3>JPN</s3><sZ>5 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Antiviral research</title><title level="j" type="abbreviated">Antivir. res.</title><idno type="ISSN">0166-3542</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Antiviral research</title><title level="j" type="abbreviated">Antivir. res.</title><idno type="ISSN">0166-3542</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS</term><term>Antiretroviral agent</term><term>Antiviral</term><term>Extract</term><term>HIV-1 virus</term><term>Human herpesvirus 1</term><term>Polygonum</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Antiviral</term><term>Antirétroviral</term><term>Virus HIV1</term><term>SIDA</term><term>Polygonum</term><term>Extrait</term><term>Herpesvirus hominis 1</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A water-soluble extract of fermented Polygonum tinctorium Aiton (Polygonaceae) called Sukumo, exhibited a potent inhibitory activity against HIV type 1 in vitro. The extract potently suppressed acute HIV-1 (Ills) infection in MT-4 cells with EC<sub>50</sub> values of 0.5 μg/ml but exhibited low cytotoxicity to MT-4 cells even at a high concentration (CC<sub>50</sub> > 1000 μg/ml). It also inhibited giant cell formation in co-cultures of HIV-infected cells and uninfected Molt-4 cells. Sukumo extract was found to interact with both the viral envelope glycoprotein and cellular receptors, thus blocking virus-cell binding and virus-induced syncytium formation. There was a good correlation between the extract's anti-HIV-1 activity and its inhibitory effects on HIV-1 binding. It also suppressed replication of herpes simplex virus type 1 in Vero cells with an EC<sub>50</sub> of 11.56 μg/ml. On the other hand, there was no appreciable activity against influenza A virus, poliovirus or SARS corona virus when tested at concentrations ranging from 3.2-400 μg/ml as shown by microscopic image analysis for cytopathic effect (CPE). Physico-chemical studies revealed that the anti-HIV activity in the extract was essentially maintained after boiling at 100 °C in 1N HCl or IN NaOH, and after treatment with 100mM NaIO<sub>4</sub>. The inhibitory activity of the extract was also not reduced after pronase digestion. The active factor in the extract is likely to be a novel compound(s) having a polyanionic substructure and a molecular weight of 10,000-50,000.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li><li>Région du Kansai</li></region><settlement><li>Kyoto</li><li>Tokyo</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Yu Zhong" sort="Yu Zhong" uniqKey="Yu Zhong" last="Yu Zhong">YU ZHONG</name></region><name sortKey="Fujii, Nobutaka" sort="Fujii, Nobutaka" uniqKey="Fujii N" first="Nobutaka" last="Fujii">Nobutaka Fujii</name><name sortKey="Honda, Gisho" sort="Honda, Gisho" uniqKey="Honda G" first="Gisho" last="Honda">Gisho Honda</name><name sortKey="Inagaki, Yoshio" sort="Inagaki, Yoshio" uniqKey="Inagaki Y" first="Yoshio" last="Inagaki">Yoshio Inagaki</name><name sortKey="Matsuda, Shinobu" sort="Matsuda, Shinobu" uniqKey="Matsuda S" first="Shinobu" last="Matsuda">Shinobu Matsuda</name><name sortKey="Shimizu, Noriko" sort="Shimizu, Noriko" uniqKey="Shimizu N" first="Noriko" last="Shimizu">Noriko Shimizu</name><name sortKey="Takeda, Tadahiro" sort="Takeda, Tadahiro" uniqKey="Takeda T" first="Tadahiro" last="Takeda">Tadahiro Takeda</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name><name sortKey="Yoshizaki, Sayaka" sort="Yoshizaki, Sayaka" uniqKey="Yoshizaki S" first="Sayaka" last="Yoshizaki">Sayaka Yoshizaki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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